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p c met  (Cell Signaling Technology Inc)


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    Structured Review

    Cell Signaling Technology Inc p c met
    P C Met, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 538 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/phospho+met/Phospho-Met+(Tyr1234%2F1235)+XP+Rabbit+mAb/pm41924840-43-52-55
    Average 96 stars, based on 538 article reviews
    p c met - by Bioz Stars, 2026-09
    96/100 stars

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    Related Articles

    Membrane:

    Article Title: Therapeutic effects of conditioned medium of immortalized dental pulp stem cells from human exfoliated deciduous teeth against experimental autoimmune neuritis.
    Article Snippet: The samples were separated by SDS-PAGE under reducing conditions and transferred to polyvinylidene difluoride membrane (Merck Millipore). .. The membrane was then blocked, and probed with antibodies against the following molecules: MBP (Catalog # ABN912, Merck Millipore), EGR2 (Clone # EPR4004, abcam), c-JUN (Clone # G-4, Santa Cruz), phospho-MET (Tyr1234/1235, Catalog # 3126, Cell Signaling), MET (Ab-1003, Catalog # SAB4300599, Merck Millipore), phospho-p44/42 MAPK ERK1/2 (Thr202/Tyr204, Catalog #9101, Cell Signaling), ERK1/2 (Clone #137F5, Cell signaling), phospho-AKT (Ser473, Catalog #9271, Cell Signaling), AKT (Catalog # 9272, Cell Signaling), phospho-ErbB2 (Tyr1196, Clone # D66B7, Cell Signaling), phosphoErbB3 (Tyr1289, Clone # 21D3, Cell Signaling), phospho-ErbB4 (Tyr984, Catalog # 3790, Cell Signaling), or β-actin (Clone # C4, Santa Cruz). .. Then, the membrane was incubated with an appropriate secondary antibody conjugated to horseradish peroxidase, and visualized with the enhanced chemiluminescence detection system (Cytiva) according to the manufacturer’s instructions.

    Article Title: Therapeutic effects of conditioned medium of immortalized dental pulp stem cells from human exfoliated deciduous teeth against experimental autoimmune neuritis
    Article Snippet: The samples were separated by SDS-PAGE under reducing conditions and transferred to polyvinylidene difluoride membrane (Merck Millipore). .. The membrane was then blocked, and probed with antibodies against the following molecules: MBP (Catalog # ABN912, Merck Millipore), EGR2 (Clone # EPR4004, abcam), c-JUN (Clone # G-4, Santa Cruz), phospho-MET (Tyr1234/1235, Catalog # 3126, Cell Signaling), MET (Ab-1003, Catalog # SAB4300599, Merck Millipore), phospho-p44/42 MAPK ERK1/2 (Thr202/Tyr204, Catalog #9101, Cell Signaling), ERK1/2 (Clone #137F5, Cell signaling), phospho-AKT (Ser473, Catalog #9271, Cell Signaling), AKT (Catalog # 9272, Cell Signaling), phospho-ErbB2 (Tyr1196, Clone # D66B7, Cell Signaling), phospho-ErbB3 (Tyr1289, Clone # 21D3, Cell Signaling), phospho-ErbB4 (Tyr984, Catalog # 3790, Cell Signaling), or β-actin (Clone # C4, Santa Cruz). .. Then, the membrane was incubated with an appropriate secondary antibody conjugated to horseradish peroxidase, and visualized with the enhanced chemiluminescence detection system (Cytiva) according to the manufacturer’s instructions.

    other:

    Article Title: Targeting the Met-RIPK1 signaling axis to enforce apoptosis and necroptosis in colorectal cancer
    Article Snippet: phospho-Met , Cell Signaling , 3077.

    Western Blot:

    Article Title: Distinct cMET inhibitors uncover pharmacological heterogeneity in SHH medulloblastoma cell lines
    Article Snippet: .. The following primary antibodies (Cell signalling, UK) were used for the immunoblot detection: MET (#4562, 1:1000), phospho-MET (#3077, 1:1000), p44/p42 MAPK (#4695, 1:1000), phospho-p44/42 MAPK (#9101, 1:1000), AKT (#9272, 1:1000), phospho-AKT (#4060. ..



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    Migration, invasion, and signaling adaptations in 786-O cells under chronic cabozantinib exposure. (A) Transwell migration assay of Par and Chr 786-O cells. Migrated cells were fixed, crystal-violet stained, and quantified using ImageJ. (B) Matrigel invasion assay performed using the same cell model and quantification workflow as in (A). Invasion was assessed following 6 h incubation through Matrigel-coated inserts. (C) Immunoblot analysis of selected signaling nodes in Par and Chr 786-O cells, including <t>MET,</t> phospho-MET (Y1234/1235), ERK1/2, phospho-ERK, c-Jun, <t>phospho-c-Jun</t> <t>(S63),</t> HSPB1, and phospho-HSPB1 (S82). β-actin served as loading control. (D) Network representation of Chr-associated phosphosites mapped to adhesion- and stress-associated signaling modules based on the annotation-enrichment analysis. Data are shown as mean ± standard deviation (SD) from three independent experiments. Statistical significance was determined using an unpaired two-tailed Student’s t-test; #p<0.05; *p<0.01.
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    Migration, invasion, and signaling adaptations in 786-O cells under chronic cabozantinib exposure. (A) Transwell migration assay of Par and Chr 786-O cells. Migrated cells were fixed, crystal-violet stained, and quantified using ImageJ. (B) Matrigel invasion assay performed using the same cell model and quantification workflow as in (A). Invasion was assessed following 6 h incubation through Matrigel-coated inserts. (C) Immunoblot analysis of selected signaling nodes in Par and Chr 786-O cells, including <t>MET,</t> phospho-MET (Y1234/1235), ERK1/2, phospho-ERK, c-Jun, <t>phospho-c-Jun</t> <t>(S63),</t> HSPB1, and phospho-HSPB1 (S82). β-actin served as loading control. (D) Network representation of Chr-associated phosphosites mapped to adhesion- and stress-associated signaling modules based on the annotation-enrichment analysis. Data are shown as mean ± standard deviation (SD) from three independent experiments. Statistical significance was determined using an unpaired two-tailed Student’s t-test; #p<0.05; *p<0.01.
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    Image Search Results


    Migration, invasion, and signaling adaptations in 786-O cells under chronic cabozantinib exposure. (A) Transwell migration assay of Par and Chr 786-O cells. Migrated cells were fixed, crystal-violet stained, and quantified using ImageJ. (B) Matrigel invasion assay performed using the same cell model and quantification workflow as in (A). Invasion was assessed following 6 h incubation through Matrigel-coated inserts. (C) Immunoblot analysis of selected signaling nodes in Par and Chr 786-O cells, including MET, phospho-MET (Y1234/1235), ERK1/2, phospho-ERK, c-Jun, phospho-c-Jun (S63), HSPB1, and phospho-HSPB1 (S82). β-actin served as loading control. (D) Network representation of Chr-associated phosphosites mapped to adhesion- and stress-associated signaling modules based on the annotation-enrichment analysis. Data are shown as mean ± standard deviation (SD) from three independent experiments. Statistical significance was determined using an unpaired two-tailed Student’s t-test; #p<0.05; *p<0.01.

    Journal: Cancer Genomics & Proteomics

    Article Title: Timescale-dependent Phosphoproteomic Remodeling and Motility-associated Adaptation under Chronic Cabozantinib Exposure in Renal Cell Carcinoma

    doi: 10.21873/cgp.20576

    Figure Lengend Snippet: Migration, invasion, and signaling adaptations in 786-O cells under chronic cabozantinib exposure. (A) Transwell migration assay of Par and Chr 786-O cells. Migrated cells were fixed, crystal-violet stained, and quantified using ImageJ. (B) Matrigel invasion assay performed using the same cell model and quantification workflow as in (A). Invasion was assessed following 6 h incubation through Matrigel-coated inserts. (C) Immunoblot analysis of selected signaling nodes in Par and Chr 786-O cells, including MET, phospho-MET (Y1234/1235), ERK1/2, phospho-ERK, c-Jun, phospho-c-Jun (S63), HSPB1, and phospho-HSPB1 (S82). β-actin served as loading control. (D) Network representation of Chr-associated phosphosites mapped to adhesion- and stress-associated signaling modules based on the annotation-enrichment analysis. Data are shown as mean ± standard deviation (SD) from three independent experiments. Statistical significance was determined using an unpaired two-tailed Student’s t-test; #p<0.05; *p<0.01.

    Article Snippet: The following primary antibodies were used: β-actin (GTX109639; GeneTex, Hsinchu, Taiwan, ROC), HSPB1 (ab5579; Abcam, Cambridge, UK), ERK1 (sc-93; Santa Cruz Biotechnology, Dallas, TX, USA), phospho-ERK (sc-7383; Santa Cruz), c-Jun (sc-1694; Santa Cruz), MET (#4560; Cell Signaling Technology, Danvers, MA, USA), phospho-MET (#3077; Cell Signaling Technology), phospho-c-Jun S63 (#9810; Cell Signaling Technology), and phospho-HSPB1 S82 (#2401; Cell Signaling Technology).

    Techniques: Migration, Transwell Migration Assay, Staining, Invasion Assay, Incubation, Western Blot, Control, Standard Deviation, Two Tailed Test